| E Frontier | Tokyo | 1999 | software industry |
| EA Bright Light | Guildford | 1995 | — |
| EA Mobile | Los Angeles | 2004 | video game industry |
| EA Montreal | Montreal | 2004 | video game industry |
| EA Orlando | Orlando | 1994 | video game industry |
| EA Romania | Bucharest | 2005 | — |
| EA Sports Big | — | 2000 | video game industry |
| Eagle Dynamics | Moscow | 1991 | video game industry |
| East Japan Railway Company | JR East Headquarters Building | April 1987 | rail transport |
| East Side Games | Vancouver | 2011 | video game industry |
| East Technology Corp. | — | — | video game industry |
| Eat Sleep Play | Salt Lake City | 2007 | video game industry |
| Eclipse Games | — | — | video game industry |
| Eclipse Software | — | 1999 | video game industry |
| Ecole Software | Hyōgo Prefecture | March 31, 1989 | video game industry |
| Edge Games | Pasadena | 1990 | video game industry |
| Edia | Hitotsubashi | April 9, 1999 | — |
| Edu-Ware | Agoura Hills | 1979 | video game industry |
| EggNut | — | 2017 | video game industry |
| Egmont International Holding | Copenhagen | September 5, 1984 | media conglomerate |
| Egosoft | Würselen | 1988 | video game industry |
| Eidos Interactive | London | 1990 | — |
| Eidos Interactive | London | 1988 | — |
| Eko Software | Choisy-le-Roi | January 10, 1999 | video game industry |
| Electric Square | Brighton | 2015 | — |
| Electronic Arts | Redwood Shores, Decatur | May 28, 1982 | video game industry |
| Electronic Zoo | Stroud | — | — |
| Elephant Games | Yoshkar-Ola | 2003 | video game industry |
| ELF Corporation | Tokyo | April 27, 1989 | video game industry |
| Elf Games | Brescia | 2014 | video game industry |
| Embark Studios | Stockholm | November 2018 | video game industry |
| Ember Entertainment | Seattle | 2011 | video game industry |
| Empire Interactive | London | 1987 | — |
| Empty Clip Studios | San Diego | July 2007 | video game industry |
| Encore, Inc. | Los Angeles | 1994 | — |
| EndlessFluff Games | New York City | — | video game industry |
| Engine Software | Doetinchem | 1995 | video game industry |
| English Software | Manchester | 1982 | — |
| Enigma Variations | — | — | — |
| EnjoyUp | Barcelona | — | video game industry |
| Enlight Software | Hong Kong | 1993 | video game industry |
| Enodo Games | — | 2014 | video game publishing |
| Ensemble | — | — | — |
| EnsenaSoft | — | — | — |
| Enterbrain | Tokyo | 2000 | video game industry |
| Entergram | Kita-ku | August 5, 1999 | video game industry |
| Entertainment Software Publishing | — | 1997 | video game industry |
| Envision Entertainment | Ingelheim am Rhein | October 8, 2013 | — |
| Envoidant Studios | — | — | — |
| EpiXR Games | — | May 2019 | video game industry |
| Epoch | Drive Slow | 1958 | video game industry |
| Epoch Co. | Taitō-ku | May 6, 1958 | video game industry |
| Epsitec SA | — | 1987 | — |
| Erbe Software S.A. | — | 1984 | video game industry |
| ERE Informatique | Vallauris | 1983 | video game industry |
| Escu:de | — | — | video game industry |
| ETH Zurich | ETH Zurich main building | October 16, 1855 | — |
| Etude | — | — | video game industry |
| Euphoria Games | Kaluga | 2019 | video game industry |
| Euphoric Brothers | — | — | video game industry |
| Eurocom | Derby | October 1988 | — |
| Europäisches Hansemuseum | — | 2015 | — |
| Everplay Interactive | — | — | video game industry |
| Evil Empire | Bordeaux | January 31, 2019 | video game publishing |
| Evolution Studios | Runcorn | 1999 | — |
| ExA-Arcadia | — | 2019 | — |
| Exakt Entertainment | Los Angeles | 1999 | video game industry |
| Exidy | Sunnyvale | 1974 | video game industry |
| Exient Entertainment | Oxford | 2001 | — |
| Expansive Worlds | — | — | video game industry |
| Explosm | — | 2009 | — |
| Extra Mile Studios | — | — | — |
| Extremely OK Games | Vancouver | April 16, 2018 | video game industry |
| Eyedentity Games | Seoul | 2007 | online game |
| École polytechnique fédérale de Lausanne | — | 1969 | — |